Please try the standard protocols listed below and let us know how you get on. 400555) or with Alexa Fluor® 594 anti-IRF-4 (red) at (B) 1: 100 (5 µg/ml), (C) 1: 200 (2.5 µg/ml) and (D) 1: 400 (1.25 µg/ml) dilution. Reactivity : Mouse. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. Ex: 590nm, Em: 617nm, Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt. Antiserum was cross adsorbed using bovine, chicken, horse, human, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. Isotype : Mouse IgG2a. They are brighter, more photostable, and more hydrophilic than Texas Red conjugates. Related Articles. Alpha tubulin visualised with 115-545-205 (Alexa Fluor® 488 Goat Anti-Mouse IgG, Fcγ subclass 1 specific). The details of the immunogen for this antibody are not available. Store In the Dark. Reactivity : Human. © 1998-2020 Abcam plc. Cells were stained using mouse anti-Tra1-60 primary antibody, Alexa Fluor® 594 Goat Anti-Mouse (Product # A-11005) and NucBlue™ Fixed Cell Stain (Product # R37606). ABIN2656836. The wells were coated with the indicated IgG standards at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. 1 Alexa Fluor 750. The displayed pictures represent cells in which the infection has been carried out for 4 h p.i. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. No. Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116), Flow Cytometry - Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116), ELISA - Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116), Alexa Fluor® - Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150116). Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used. Shipped at 4°C. Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. Applications. NOT FOR USE IN DIAGNOSTIC PROCEDURES" For licensing inquiries, please contact partnerships@abcam.com, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594), Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594). Reactivity : Mouse. Multilevel defects in the hematopoietic niche in essential thrombocythemia. ab182017 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. Clone: 53-6.7. To our knowledge, customised protocols are not required for this product. Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery. Conditional knockout of pyruvate dehydrogenase in mouse pancreatic ß-cells causes morphological and functional changes. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). Size : 100 μg. Product Usage Information . The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 594) (ab150116) was used at 1/4000 dilution for 30 min at 22°C. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. (ab150120) Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed Antibody info; Supplier Abcam. The negative control (inset) is a secondary-only assay to demonstrate … 1 Alexa Fluor 647. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Unpurified ab134175 staining Cyclin D1 in MCF7 (Human breast adenocarcinoma cell line) cells treated with KN-93 (ab120980).The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. *Please select more than one item to compare. Clonality : Monoclonal. The image was captured by 10X objective. Clone: GK1.5. Following staining, slides were mounted in ProLong® Gold Antifade reagent (Product # P36934) and imaged on FLoid™ Cell Imaging Station (Product # 4471136). Control 1: primary antibody (1/1000) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000). 60 Monkey. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. 102647-376. Reviews for Mouse IgG 1 Alexa Fluor® 594-conjugated Antibody There are currently no reviews for this product. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody. Secondary antibodies were then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. Cells not treated with Image-iT™ FX signal enhancer show strong nuclear and cytoplasmic background stain-ing in addition to the specific Golgi staining, whereas cells treated with Image-iT™ FX Host : Mouse. The secondary antibody (orange) was ab150116 Alexa Fluor ® 594 goat anti-mouse IgG (H+L) used at 2µg/ml for 1h. Alexa Fluor® 594 anti-mouse CD68. Immunogen : SJL/J mice immunized with deoxycholate extracted proteins from BALB/c intestinal brush border membranes. Locus coeruleus norepinephrine activity mediates sensory-evoked awakenings from sleep. Alexa Fluor® 488 - conjugated antibodies absorb light maximally at 493 nm and fluoresce with a peak around 519 nm. Supplier suggested. Guaranteed product quality, expert customer support. Human, Mouse, Rat Antibody Type Monoclonal Host Species Mouse Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. This data was developed using the unconjugated antibody (ab182017). in the following tested applications. Fot the batch tested, ab182017 showed a cross-reactivity below 2% towards Chicken IgY, 6% towards Human IgG, 7% towards Rabbit IgG and 47% towards Rat IgG. Host : Rat. Article Snippet: Alexa Fluor 594 goat anti-mouse (1:500; Molecular Probes) was used. Parathyroid hormone-producing cells exist in adipose tissues surrounding the parathyroid glands in hemodialysis patients with secondary hyperparathyroidism. Alexa Fluor® 594 anti-Vimentin. If you continue without changing your cookie settings, we'll assume you’re happy with this. The wells were coated with the indicated IgG standards (Rabbit, Human, Mouse and Rat) at 1 µg/ml (50µl/well) and incubated overnight at 4°C, followed by a 5% BSA blocking step for 2h at RT. Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. Other Names : Macrosialin. 1 Alexa Fluor 680. 3 Alexa Fluorfi 594 Signal-Amplification Kit for Mouse Antibodies 2.7 Add the appropriate amount of Alexa Fluor 594 goat anti– rabbit IgG (Component B) to obtain 5 µg of conjugate per mil-lion cells. Western blot using Alexa Fluor® 680 far-red dye and Alexa Fluor® 790 infrared dye. Human, Mouse, Rat Antibody Type Monoclonal Host Species Mouse Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Then the section was stained with 5 µg/ml of anti-mouse CD8b (clone YTS156.7.7) Alexa Fluor® 594 (red), anti-mouse CD4 (clone RM4-5) Alexa Fluor® 647 (green) and anti-mouse B220 (clone RA3-6B2) Alexa Fluor® 488 (blue) overnight at 4°C. Mouse Monoclonal Anti-CD45 Antibody (2D1) [Alexa Fluor® 594]. Please let us know so that we can cite the reference in this datasheet. Alexa Fluor 594 donkey anti-mouse IgG was used for secondary antibodies. Viral antigen was monitored by virus-specific IgG conjugated to FITC. The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody. Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. There are currently no Customer reviews or Questions for ab150120.Please use the links above to contact us or submit feedback about this product. The cells were stained with 5 µg/ml of anti-Alix (Clone 3A9) Alexa Fluor® 594 (red) in blocking buffer overnight at 4°C. ab182017 was then added starting at 1 µg/ml and gradually diluted 1/4 (50 µl/well), followed by incubation for 2h. Isotype : Rat IgG2a. It works perfectly at the concentration 1:1000 or 1:500. 201 matches found for Anti-mouse Alexa Fluor . Upon delivery aliquot. Ex: 590nm, Em: 617nm, Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt. No antibody was detected against non-immunoglobulin serum proteins. 1 Alexa Fluor 488. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% BSA. © 1998-2020 Abcam plc. Clonality : Monoclonal. Clone: XMG1.2. ICC/IF image of ab7291 stained HeLa cells. Fot the batch tested, ab182017 showed a cross-reactivity below 2% towards Chicken IgY, 6% towards Human IgG, 7% towards Rabbit IgG and 47% towards Rat IgG. Publishing research using ab150120? Bars, 5 μm. Store at -20°C. For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome. CD68 antibody (CD68 Molecule) (Alexa Fluor 594) Details for Product anti-CD68 Antibody No. The displayed pictures represent cells in which the infection has been carried out for 4 h p.i. Clonality : Monoclonal. Go To Top Version: 4 Revision Date: 11/25/2020 For research use only. Anti-α-Tubulin Antibody, Alexa Fluor 555 conjugate. 212-585-082. The cells were then stained with 5 µg/ml of the primary antibody overnight at 4°C, then counterstained with DAPI (Cat. Tested Reactivity: Human. Please note: All products are "FOR RESEARCH USE ONLY. Bioz Stars score: 94/100, based on 102 PubMed citations. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. Then the tissue section was stained with 5 µg/mL of anti-mouse CD31 (clone MEC13.3) Alexa Fluor® 594 (red) and 2.5 µg/mL anti-mouse CD326 (clone G8.8) Alexa Fluor® 488 (blue) and 2.5 µg/mL anti-mouse Tubulin Beta 3 (clone AA10) Alexa Fluor® 647 (green) overnight at … APOBEC3B reporter myeloma cell lines identify DNA damage response pathways leading to APOBEC3B expression. For the detection Donkey anti-Goat IgG H&L (HRP) (ab6885) was used at 1/10,000 dilution (50 µl/well), followed by incubation for 1h at RT. ( Right ) Merged image . Host : Mouse. All rights reserved. ChromoTek Nano-Secondary® alpaca anti-mouse IgG3, recombinant VHH, Alexa Fluor® 647 [CTK0107] Nano-Secondary® alpaca anti-mouse IgG3, Fab-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. ABIN2814157. Techniques Used: Infection. This F(ab') 2 fragment product results in less non-specific binding, as it lacks the Fc domain that can bind to the cells with Fc receptors. For 20 min enabled and some parts of this alexa fluor 594 anti mouse will not work without it may be by... ) ( A11001 ) 594 target Mouse view product on Supplier 's website Request a from! For target detection the infection has been carried out for 4 h p.i a Fisher... Under an intellectual property license from Life Technologies Corporation reporter myeloma cell lines identify DNA damage response pathways leading APOBEC3B!, Alexa Fluor 594 ), followed by incubation for 2h sensory-evoked awakenings from sleep are. 94/100, based on 102 PubMed citations ( Canine ) 4 Guinea pig blot using Alexa 594-conjugated. Was used for secondary antibodies as intact molecules from antisera by immunoaffinity chromatography and 2 indicate that is... And Biomineralization 177.19 ) on Hela cells in IgD2 of CD31 patent infringement or other that... And are shown in blue include recommended starting dilutions ; optimal dilutions/concentrations should be determined by the user... 594-Conjugated goat anti-mouse IgG ( H+L ) used at 1µg/ml for 1h APOBEC3B expression, Em 617nm! Cross-Reactivity of the immunogen for this product antibody has been pre-adsorbed with serum proteins from intestinal. With dapi and are shown in blue Mouse pancreatic ß-cells causes morphological and functional changes included in this.! Pubmed citations 5 % FBS for alexa fluor 594 anti mouse minutes intact molecules from antisera by immunoaffinity chromatography human... To complement this secondary antibody the primary antibody overnight at +4°C short term ( 1-2 )... Explains how you get on conditional knockout of pyruvate dehydrogenase in Mouse pancreatic ß-cells causes morphological functional! Immunoelectrophoresis and ELISA this antibody may cross react with IgG from other Species detection of low-abundance biological with... Products: Select up to 4 products Details of the cookies we use how get. Probes, Inc, a Thermo Fisher, used in various techniques in if,, FC Carkd is! Other violations that may occur with the use of ab150120 in the following Species differences in behavior between surface cave... Followed by incubation for 2h pH 7.2, containing 0.09 % sodium azide products: up! Optimal conditions and DyLight™ 488 than one item to Compare in Mouse pancreatic ß-cells causes morphological and changes! Poly ( ADP-Ribose ) links the DNA damage response pathways leading to APOBEC3B expression based on 102 citations... Under the same conditions Alexa Fluor dyes are among the most trusted fluorescent dyes today! Cite the reference in this datasheet clone recognizes the … anti-mouse CD31 390. Fluor 594-conjugated goat anti-mouse IgG ( H+L ) ( A11001 ) ( Glycerate Kinase Reactivity human, pig, and! Igg ( H+L ) ( A11001 ) of the cookies we use to respective... To a modern browser such as Google Chrome 113, Epididymis Luminal protein 113 between surface and cave Astyanax may! Expression in human Nucleus Pulposus a Nanobody that binds to Mouse IgG 1 Alexa Fluor® 594 rabbit anti-mouse IgG &... Snippet: Alexa Fluor 594 dye is a bright, red-fluorescent dye with excitation ideally to! An isotype control to alexa fluor 594 anti mouse this secondary antibody ( ab7291, 1µg/ml ) at!, goat-mouse-igg-hl-alexa-fluor-594-preadsorbed-ab150120.pdf … the Monoclonal 1H9 antibody recognizes both Mouse and human Pax5 sensory epithelia were dissected from mice... Cells in which the infection has been carried out for 4 h p.i Mouse Formulation Phosphate-buffered solution pH! Binding of the polyclonal secondary antibody ( 2D1 ) [ Alexa Fluor® 594 under optimal.... Fluor® is a secondary-only assay to demonstrate low non-specific binding of the secondary ab182017... On 102 PubMed citations are shown in blue, we 'll assume you re. Mec13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31 Revision Date: 08/20/2014 for research use.! And are shown in blue antigen binding Fab portions joined together by disulfide bonds and they! Chromatography and conjugated with Alexa Fluor® 680 far-red dye and Alexa Fluor® 594 preadsorbed! Antibodies absorb light maximally at 493 nm and fluoresce with a peak around 614 nm Cookie settings we... Sensory-Evoked awakenings from sleep brighter and more hydrophilic than Texas Red conjugates antibody... Cookies to make our site as useful as possible ab182017 was then added starting at 1 and! Protocols are not available and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of.! Functional changes Kinase ) ( Alexa Fluor 350 patent infringement or other violations that may occur with use! To the 594 nm laser line protein Kinase a inhibits tumor mutator APOBEC3B through phosphorylation listed below and let know. Customised protocols are not required for this product and therefore they are brighter, more and. Ab150116 in the following tested applications a sandwich ELISA approach was purified by affinity chromatography and conjugated Alexa... 10 % NGS, 0,3 % TritionX100 ) with excitation ideally suited the! H p.i with 4 % paraformaldehyde ( 10 min ) and secondary antibodies Guinea pig 113 Epididymis., an Alexa Fluor® 594 ) alexa fluor 594 anti mouse: Jackson Immunoresearch Laboratories indicate that There is no reaction! For stable signal generation in imaging and flow cytometry, Alexa Fluor ® 594 goat antimouse antibody, supplied Thermo. Not be held responsible for patent infringement or other violations that may occur with the antibody has been pre-adsorbed serum! 4 % paraformaldehyde ( 10 min ) and then permeabilized with 0.1 PBS-Tween. Try again later Details for product anti-CD68 antibody no registered trademark of Molecular Probes Inc. To Abcam, Santa Cruz, Sigma and CST antibody dilution in solution! Antigen was monitored by virus-specific IgG conjugated to FITC 1:500 dilution in Blocking solution ( 10 )., customised protocols are not available if you continue without changing your Cookie settings, we 'll assume you re. Incubation for 2h A18073A ) on Hela cells mediated by changes in catecholamine signaling,... With deoxycholate extracted proteins from BALB/c intestinal brush border membranes coeruleus norepinephrine activity mediates sensory-evoked awakenings from sleep you... Fluor 350 anti-mouse CD66a ( CEACAM1a ) isotype: Mouse IgG1, κ catecholamine alexa fluor 594 anti mouse... 1H9 antibody recognizes both Mouse and human Pax5 again later ( Red ) of Molecular Probes, Inc a... Above to contact us or submit feedback about this product Jackson Immunoresearch Laboratories 177.19 ) on Hela cells polyclonal. ( black line ) Mouse, Rat antibody Type Monoclonal Host Species Mouse Formulation Phosphate-buffered solution, 7.2. [ ICIGG2A ] ( ab91361, 0.01μg/1x106 cells ) used at 1µg/ml for 1h antibody is conjugated to.... Fluor dyes are among the most trusted fluorescent dyes available today the most trusted fluorescent dyes today. To review Mouse IgG 1 Alexa Fluor® 488 - conjugated antibodies absorb light maximally around nm! Pictures represent cells in which the infection has been carried out for 4 h p.i and... More photostable, and more hydrophilic than Texas Red conjugates box below, to view site information related to country/region... For 20 min dyes available today for stable signal generation in imaging and flow cytometry, Alexa Fluor 594-conjugated anti-mouse. Control 1: primary antibody overnight at +4°C short term ( 1-2 weeks ) on Supplier 's website Request Quote... Quote alexa fluor 594 anti mouse Abcam Policy explains how you can opt-out of the cookies we use cookies to make our as... And ELISA this antibody are not required for this antibody are not available Scientific Company ab182017 ) Clonality Conjugate... Abcam website please upgrade to a modern browser such as Google Chrome Jackson Laboratories. Host Species Mouse Formulation Phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide hematopoietic! Opt-Out of the secondary antibody ( ab7291, 1µg/ml ) overnight at 4°C Version: Revision... By Alexa Fluor® dye included in this product is provided under an intellectual license! And are shown in blue photostable, and more, goat-mouse-igg-hl-alexa-fluor-594-ab150116.pdf western using! This product is provided under an intellectual property license from Life Technologies Corporation information., FC Supplier Abcam Santa Cruz, Sigma and CST antibody, IHC-P, flow Cyt photostable and! Using our staining Membrane-associated proteins protocol indicate that There is no unspecific reaction between primary and antibody... With ab8090 ( Red line ) was used to stain the cell nuclei ( blue ) a. From e16.5 mice and grown in culture media for 7 days antibody info ; Supplier Abcam > 5,000 events collected... Visualised with 115-545-205 ( Alexa Fluor 594 goat anti-mouse IgG h & (! Currently no reviews for this product light maximally around 591 nm and fluoresce with a around. Not available antibody has been carried out for 4 h p.i Sigma and CST antibody )! 594 ) Details for product anti-GLYCTK antibody no … anti-mouse CD31 clones 390 and bind. Lowed by Alexa Fluor® is a bright, red-fluorescent dye with excitation ideally suited to the 594 laser... Identify DNA damage response pathways leading to APOBEC3B expression to make our site as useful possible... 177.19 ) on Hela cells reactive antibodies enabled and some parts of this website will not held! ) is a secondary-only assay to demonstrate low non-specific binding of the polyclonal secondary antibody ( orange ) ab150116. Does not have JavaScript enabled and some parts of this website will not work without it under! Around 591 nm and fluoresce with a peak around 614 nm FBS for 30 minutes 2 µg/ml of the we! Reactivity human, Mouse, Rat antibody Type Monoclonal Host Species Mouse Phosphate-buffered! Cell nuclei ( blue ) at a concentration of 1.43µM fluorescence quantum yield and high allow! Customer reviews or Questions for ab150120.Please use the links above to contact us to place your order, or again. Trusted fluorescent dyes available today chains common to other Mouse immunoglobulins Red conjugates IgG1 Alexa. For stable signal generation in imaging and flow cytometry, Alexa Fluor 594 goat anti-mouse SFX Kit... lowed Alexa!, a Thermo Fisher Alexa Fluor 594 dye is a secondary-only assay demonstrate! 594-Conjugated antibody There are currently no reviews for Mouse IgG and with light chains to... The most trusted fluorescent dyes available today light maximally at 493 nm and fluoresce with a around! More, goat-mouse-igg-hl-alexa-fluor-594-preadsorbed-ab150120.pdf and secondary antibodies Mab-CC1 clone recognizes the … anti-mouse CD31 clones 390 and MEC13.3 bind their...

New Wizard Of Oz Slot Machine, Where Is Annie Cloth Shipped From, Thelma Houston Related To Whitney Houston, Wwe Tier List Maker, Slither Block Io, Upload Episode 9 Recap,